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All TrxR enzymes require an NADPH-dependent reduction step to exert their function in vivo and TrxR is a therapeutic target for cancer and infectious diseases 21,67

Secondary analysis using an ELISA based approach confirms GPx3 is substantially reduced in children affected with konzo To validate the findings obtained using proteomic-based applications, we sought to determine if the reduced abundance of GPx3 protein in konzo affected children as compared to their unaffected siblings could also be detected using an orthogonal ELISA-based approach

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The same visual staining intensities were seen in the hippocampus (not shown)